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How does a sewage treatment plant remove phosphorus and nitrogen?

Municipal plants remove nutrients in an advanced stage after solids and organic matter are handled, using biological and chemical routes that are being made more efficient.

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Covers: This page explains the biological, chemical and physical processes used to remove nitrogen and phosphorus at municipal wastewater treatment plants, including nitrification, denitrification, enhanced biological phosphorus removal and chemical precipitation. It does not cover industrial pretreatment, septic systems or drinking-water treatment.

Also answers: How is nitrogen removed from wastewater? · How do wastewater plants remove phosphorus? · Biological nutrient removal in sewage treatment · What happens to nitrogen and phosphorus in a treatment plant?

Aerial view of a water treatment plant with surrounding fields
Photo: Subhash Chand

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The short answer

Interpretation AI-prepared starting map

Municipal sewage treatment removes nitrogen and phosphorus mainly through a sequence of stages: primary treatment settles solids, secondary treatment uses biological processes to cut organic matter, and a tertiary or advanced stage adds nutrient removal and polishing. Within that framework, nitrogen is typically removed by nitrification followed by denitrification, and phosphorus by enhanced biological phosphorus removal (EBPR) and/or chemical precipitation. Recent full-scale and lab-scale work has focused on making these routes more energy-efficient and more stable, including membrane bioreactor designs, hardness-driven chemical-biological phosphorus synergy, and anammox-based nitrogen pathways.12345

What this rests on6 independent sources
  • Evidence 17
  • Interpretation 6

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In brief

  1. Nutrient removal is an advanced (tertiary) stage added to primary and secondary treatment, which handle solids and organic matter respectively.1

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  2. Nitrogen is removed by nitrification plus denitrification, with newer anammox-based and fermentation-driven configurations reaching over 93% removal on low carbon-to-nitrogen municipal wastewater.45

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  3. Phosphorus is removed by a combination of biological uptake and chemical binding, and higher water hardness shifts more phosphorus into stable calcium- and magnesium-bound minerals while favouring phosphorus-accumulating organisms.3

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  4. Membrane bioreactor design choices affect nutrient removal, chemical dosing and energy: a vibrating MBR cut effluent total nitrogen by 22%, phosphorus-removal chemical dosage by 40% and fouling-control energy by 75% versus a conventional aerated MBR in one full-scale trial.2

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  5. Removed nutrients end up in sludge, so sludge treatment and disposal choices are part of the nutrient-removal story.6

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At a glance

The picture in numbers

Live · updated just now

Step-feed sequencing batch reactor study

93.2%

93 in every 100

of nitrogen removed by a sulfide-driven partial denitrification and anammox process4
240-day continuous municipal wastewater trial

96.9%

97 in every 100

of nitrogen removed with alkaline sludge fermentation liquor under partial nitrification5
Parallel trial at 7500 m3 per day for about 800 days
  • Lower effluent total nitrogen22%
  • Lower phosphorus-removal chemical dosage40%
  • Lower fouling-control energy75%
  • Lower overall carbon footprint30%
Vibrating MBR versus conventional aerated MBR in one full-scale trial2
Phosphorus forms and microbial community tracked over time

190 days

Length of the water hardness and phosphorus removal study3

The evidence behind it

6 sources
  • Other studies and data4
  • Background2

Published in 2026

Sources on this page by kind and year
SourceKindYear
Sewage treatment (Wikipedia)BackgroundUnknown
Vibrating membrane bioreactors enhance nutrient removal and energy efficiency in municipal wastewater treatment.Other studies and data2026
Water hardness-mediated EBPR-BIPP synergistic phosphorus removal and microbial community response.Other studies and data2026
Sulfide-driven partial denitrification serves as a remedial strategy for suboptimal partial nitrification-anammox performance.Other studies and data2026
Robust nitrogen removal through simultaneous denitrification and anammox driven by alkaline sludge fermentation liquid at varying nitrification levels.Other studies and data2026
Sewage sludge treatment (Wikipedia)BackgroundUnknown

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The path through it

How it comes together

The full story · 5 chapters

01

Where nutrient removal sits in a treatment plant

AI summary:Nutrient removal is an added tertiary stage; primary and secondary stages handle solids and organic matter.

Evidence-backed

Evidence-backed: Sewage treatment aims to remove contaminants so the effluent can be discharged or reused, preventing water pollution from raw sewage. Plants range from decentralized on-site systems to large centralized networks of pipes and pump stations. Treatment commonly has two main stages, primary and secondary, and advanced treatment adds a tertiary stage with polishing processes and nutrient removal. Secondary treatment reduces organic matter, measured as biological oxygen demand, using aerobic or anaerobic biological processes.1

Interpretation

Interpretation: Nutrient removal is therefore an add-on to the core carbon-removal train: the biological secondary stage handles organic matter, while nitrogen and phosphorus are targeted by the advanced or tertiary processes described in the following sections.1

02

Removing nitrogen: nitrification, denitrification and anammox routes

AI summary:Nitrogen is removed by nitrification and denitrification, with newer anammox and fermentation-driven setups reaching high removal on low-carbon wastewater.

Evidence-backed

Evidence-backed: Conventional nitrogen removal relies on nitrification converting ammonia to nitrate and denitrification converting nitrate to nitrogen gas. A recurring problem for low carbon-to-nitrogen municipal wastewater is unstable nitrite supply and nitrate accumulation in partial nitrification-anammox (PNA) processes. One study developed a coupled suboptimal partial nitrification, sulfide-driven partial denitrification and anammox (sPN-SPDA) process in a step-feed sequencing batch reactor, introducing sulfide as an electron donor during a second feeding stage. It achieved a nitrogen removal efficiency of 93.2 ± 1.2%, with effluent total inorganic nitrogen as low as 5.1 ± 0.5 mg/L. Sulfide addition enriched sulfide-driven partial denitrification denitrifiers, particularly Thauera (16.3%), supporting conversion of nitrate to nitrite, and metagenomic analysis showed increased abundances of the nitrate-reduction genes napA and napB.4

Evidence-backed

Evidence-backed: A second approach used alkaline sludge fermentation liquor (ASFL) to drive simultaneous denitrification and anammox under varying nitrification levels. Over 240 days of continuous operation treating municipal wastewater, nitrogen removal efficiency ranged from 96.9 ± 2.0% under partial nitrification to 95.7 ± 2.4% when partial and complete nitrification coexisted. Isotope tracing and microbial analysis indicated that ASFL-supported heterotrophic denitrification was the dominant nitrogen removal pathway, while anammox remained detectable but contributed only to a limited extent under those conditions. An iron-assisted polishing step was combined with the system for effluent treatment.5

Interpretation

Interpretation: Taken together, these two studies point in the same direction — anammox-based and fermentation-driven configurations can reach high nitrogen removal on low carbon-to-nitrogen wastewater — but they disagree on how much anammox itself contributes, with one finding strong sulfide-driven partial denitrification support and the other finding heterotrophic denitrification dominant. The dominant pathway appears to depend on the specific configuration and operating conditions.45

03

Removing phosphorus: biological uptake and chemical binding

AI summary:Phosphorus is removed by biological uptake plus chemical binding, and harder water shifts it toward stable minerals while favoring phosphorus-storing organisms.

Evidence-backed

Evidence-backed: Phosphorus removal combines enhanced biological phosphorus removal (EBPR) with chemical pathways. In a study of water hardness effects, sequential extraction and speciation analysis using 31P MAS-NMR, XRD and XPS showed phosphorus transforming from soluble forms to stable calcium- and magnesium-bound forms, predominantly hydroxyapatite (HAP) and struvite (MAP), with the MAP proportion increasing at higher hardness. Under high hardness, polyphosphate kinase and polyphosphatase activities rose by 109.1% and 80.7% respectively, and the ratio of polyphosphate-accumulating organism (PAO) taxa to glycogen-accumulating organism (GAO) taxa increased from 0.24 to 2.5. Over a 190-day operation, GAO-associated taxa fell from 5.0% to 2.0%, and the phoR gene reached its highest relative abundance (0.45%) under high-hardness conditions, suggesting a link between extracellular chemical fixation and intracellular storage.3

Interpretation

Interpretation: This work frames biological and chemical phosphorus removal as synergistic rather than competing: hardness promotes precipitation of stable calcium- and magnesium-bound minerals while also favouring the microbial community that stores phosphorus intracellularly. The authors present it as a basis for optimizing EBPR in high-hardness regions.3

04

Membrane bioreactors and energy use

AI summary:A full-scale vibrating membrane bioreactor cut effluent nitrogen, phosphorus chemical dosing, and fouling-control energy compared with a conventional aerated one.

Evidence-backed

Evidence-backed: A full-scale comparison of a vibrating membrane bioreactor (VMBR) and a conventional aerated membrane bioreactor (AMBR) ran for approximately 800 consecutive days at 7500 m3 per day in parallel. The VMBR lowered effluent total nitrogen by 22% and reduced the chemical dosage for phosphorus removal by 40% compared with the AMBR. It also had a 20% higher average specific flux throughout operation. Mechanism analysis attributed this to effective, uniform shear at the membrane surface that preserved sludge floc structure and mitigated fouling. The VMBR reduced specific energy consumption for fouling control by 75% and the overall carbon footprint by 30%.2

Interpretation

Interpretation: This is the only full-scale, long-duration comparison in the current material, and it links nutrient performance directly to membrane fouling control and energy demand — a reminder that nitrogen and phosphorus removal choices interact with the plant's energy and chemical footprint.2

05

What happens to the removed nutrients and sludge

AI summary:Removed nutrients end up in sludge, so sludge treatment and disposal are part of the nutrient-removal picture.

Evidence-backed

Evidence-backed: Nutrient removal produces sludge that must itself be treated. Sludge treatment focuses on reducing weight and volume to cut transport and disposal costs and on reducing health risks, with water removal as the primary means of volume reduction and pathogen destruction often achieved by heating during thermophilic digestion, composting or incineration. Method choice depends on sludge volume and the costs of available disposal options: air-drying and composting may suit rural communities, aerobic digestion and mechanical dewatering may be preferable where land is limited, and economies of scale can favour energy recovery in metropolitan areas. Primary sludge includes settleable solids removed in primary clarifiers.6

Interpretation

Interpretation: This matters for nutrient removal because phosphorus and nitrogen captured in sludge are only truly removed from the water if the sludge pathway handles them — for example through the calcium- and magnesium-bound minerals noted in the hardness study, which end up in the sludge stream.63

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What to remember

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  1. Nitrogen is removed by nitrification plus denitrification, with newer anammox-based and fermentation-driven configurations reaching over removal on low carbon-to-nitrogen municipal wastewater.

  2. Membrane bioreactor design choices affect nutrient removal, chemical dosing and energy: a vibrating MBR cut effluent total nitrogen by , phosphorus-removal chemical dosage by 40% and fouling-control energy by 75% versus a conventional aerated MBR in one full-scale trial.

  3. Nutrient removal is an advanced (tertiary) stage added to primary and secondary treatment, which handle solids and organic matter respectively.

top view of concrete structuresUp nextHow does a sewage treatment plant work and what happens to the sludge?How does a sewage treatment plant work, and what happens to the sludge it produces?

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Sources

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  1. 1
    Sewage treatment (Wikipedia)
    WikipediaPublished Oct 10, 2026Checked Oct 11, 2026
    “Sewage treatment is a type of wastewater treatment which aims to remove contaminants from sewage to produce an effluent that is suitable for discharge to the surrounding environment or an intended reuse application, thereby preventing water pollution from raw sewage discharges. Sewage contains wastewater from households and businesses and possibly pre-treated industrial wastewater. There are a large number of sewage treatment processes to choose from. These can range from decentralized systems (including on-site treatment systems) to large centralized systems involving a network of pipes and pump stations (called sewerage) which convey the sewage to a treatment plant. For cities that have a combined sewer, the sewers will also carry urban runoff (stormwater) to the sewage treatment plant. Sewage treatment often involves two main stages, called primary and secondary treatment, while advanced treatment also incorporates a tertiary treatment stage with polishing processes and nutrient removal. Secondary treatment can reduce organic matter (measured as biological oxygen demand) from sewage, using aerobic or anaerobic biological processes.”
  2. 2
    Vibrating membrane bioreactors enhance nutrient removal and energy efficiency in municipal wastewater treatment.
    Nature communications (Lin et al.)Published Jul 20, 2026Checked Oct 11, 2026
    “Here, we report approximately 800 consecutive days of full-scale (7500 m3 d-1) parallel operation of the vibrating MBR (VMBR) and the conventional aerated MBR (AMBR) systems, systematically comparing their pollutant removal performance, long-term filtration stability, membrane fouling mechanisms, and full life cycle environmental impacts. Results showed that the VMBR demonstrated enhanced nutrient removal, lowering effluent total nitrogen by 22% and reducing the chemical dosage for phosphorus removal by 40% compared with the AMBR. It also exhibited enhanced fouling resistance, with a 20% higher average specific flux than that of the AMBR throughout the operation. Mechanism analysis revealed that the VMBR generated effective, uniform shear at the membrane surface while preserving the sludge floc structure, thereby mitigating membrane fouling. Consequently, the VMBR reduced specific energy consumption for fouling control by 75% and the overall carbon footprint by 30%. We validate that VMBRs represent a scalable, energy-efficient and sustainable technology for future wastewater treatment.”
  3. 3
    Water hardness-mediated EBPR-BIPP synergistic phosphorus removal and microbial community response.
    Journal of environmental management (Zhang et al.)Published Sep 21, 2026Checked Oct 11, 2026
    “Sequential extraction and speciation analysis (31P MAS-NMR, XRD, XPS) revealed phosphorus transformation from soluble to stable calcium- and magnesium-bound forms, predominantly hydroxyapatite (HAP) and struvite (MAP), with the MAP proportion increasing at higher hardness. Under high hardness, PPK and PPX activities increased by 109.1% and 80.7%, respectively, and the relative abundance ratio of PAO/DPAO-associated taxa to GAO/DGAO-associated taxa increased from 0.24 to 2.5. A 190-day operation confirmed the synergistic interactions between BIPP and EBPR. Microbial analysis revealed that the relative abundance of GAO/DGAO-associated taxa decreased from 5.0% to 2.0%, while the phoR gene showed its highest relative abundance (0.45%) under high-hardness conditions, suggesting a potential association between extracellular chemical fixation and intracellular storage. This study elucidates the hardness-driven synergistic mechanisms, providing a theoretical basis for EBPR optimization in high-hardness regions.”
  4. 4
    Sulfide-driven partial denitrification serves as a remedial strategy for suboptimal partial nitrification-anammox performance.
    Bioresource technology (Wang et al.)Published Sep 9, 2026Checked Oct 11, 2026
    “The partial nitrification-anammox (PNA) process applied to low C/N municipal wastewater treatment is often constrained by unstable nitrite supply and nitrate accumulation. In this study, a coupled suboptimal partial nitrification, sulfide-driven partial denitrification, and anammox (sPN-SPDA) process was developed in a step-feed sequencing batch reactor, in which sulfide was introduced as an electron donor during the second feeding stage. A high nitrogen removal efficiency of 93.2 ± 1.2 % was achieved, with effluent total inorganic nitrogen reduced to as low as 5.1 ± 0.5 mg/L. Microbial community analysis demonstrated the enrichment of sulfide-driven partial denitrification (SPD)-related denitrifiers after sulfide addition, particularly Thauera (16.3 %), which supported the conversion of NO3--N to NO2--N. Metagenomic analysis showed increased abundances of nitrate-reduction genes napA and napB, consistent with improved nitrite availability for anammox. Overall, this study provides a promising strategy to alleviate nitrite limitation caused by suboptimal partial nitrification and to achieve advanced nitrogen removal from low C/N municipal wastewater.”
  5. 5
    Robust nitrogen removal through simultaneous denitrification and anammox driven by alkaline sludge fermentation liquid at varying nitrification levels.
    Bioresource technology (Li et al.)Published Aug 1, 2026Checked Oct 11, 2026
    “Here, a stable simultaneous denitrification-anammox system driven by alkaline sludge fermentation liquor (ASFL) under varying nitrification levels (PN, complete nitrification, and their coexistence) has been presented. Through a 240-day continuous operation treating municipal wastewater, this system achieved high nitrogen removal efficiency, ranging from 96.9 ± 2.0% under PN to 95.7 ± 2.4% under coexistence of PN and complete nitrification. Isotope tracing and microbial analyses indicated that ASFL-supported heterotrophic denitrification was the dominant nitrogen removal pathway, whereas anammox activity remained detectable but contributed only to a limited extent under the investigated conditions. Metagenomic analysis revealed functional shifts in nitrogen metabolism while the core carbon metabolic potential remained largely conserved. Combined with an optimized iron-assisted strategy for effluent polishing, this ASFL-driven simultaneous denitrification-anammox framework provides a sustainable, circular pathway for energy-efficient nitrogen removal, addressing the inherent vulnerability of mainstream PN applications.”
  6. 6
    Sewage sludge treatment (Wikipedia)
    WikipediaPublished Oct 10, 2026Checked Oct 11, 2026
    “Sewage sludge treatment describes the processes used to manage and dispose of sewage sludge produced during sewage treatment. Sludge treatment is focused on reducing sludge weight and volume to reduce transportation and disposal costs, and on reducing potential health risks of disposal options. Water removal is the primary means of weight and volume reduction, while pathogen destruction is frequently accomplished through heating during thermophilic digestion, composting, or incineration. The choice of a sludge treatment method depends on the volume of sludge generated, and comparison of treatment costs required for available disposal options. Air-drying and composting may be attractive to rural communities, while limited land availability may make aerobic digestion and mechanical dewatering preferable for cities, and economies of scale may encourage energy recovery alternatives in metropolitan areas. Sludge is mostly water with some amounts of solid material removed from liquid sewage. Primary sludge includes settleable solids removed during primary treatment in primary clarifiers.”

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  1. Version 2Oct 11, 2026Live now

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  • “Where nutrient removal sits in a treatment plant” rests on one independent source

    A second, independent source that confirms or challenges it would make this part more reliable.

  • “Removing phosphorus: biological uptake and chemical binding” rests on one independent source

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  • “Membrane bioreactors and energy use” rests on one independent source

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Open questions

  • How much of mainstream nitrogen removal can anammox realistically contribute, given that one study found it detectable but limited while another found strong support for sulfide-driven partial denitrification?

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  • Do the hardness-driven EBPR benefits seen over 190 days hold at other plants and water hardness ranges, and how do they interact with chemical precipitation dosing?

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  • How do the vibrating membrane bioreactor's nitrogen, phosphorus, energy and carbon benefits translate to plants of different sizes and to retrofits of existing membrane bioreactors?

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  • What happens to phosphorus bound as hydroxyapatite or struvite in the sludge stream, and can it be recovered?

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